Part:BBa_K2197301

GFP downstream a repressor operator site

Design of BBa_K2197300

Expression of a strong repressor (mUTS)

Operator site and GFP

Expression without uric acid (-UA)

mUTS binds with HucO and repress the expression of downstream GFP.

expression with uric acid (+UA)

mUTS dissociates with HucO to different extents according to the concentration of uric acid, thus expressing downstream GFP at different levels.

BBa_K2197301 - encoding hucO(BBa_K2197303) and GFP(E0040) gene

This is a composite part encoding hucO(BBa_K2197303) and GFP(E0040) gene. The circuit uses a bacterial transcriptional repressor (HucR) that binds a DNA sequence motif (hucO) in the absence of uric acid. When uric acid is present, HucR dissociates from hucO motif, thereby allowing expression of a downstream GFP gene. It is hoped that the expression of the downstream GFP gene is regulated by the concentration of uric acid in a proportional manner.

When put in a composite part, the part helps express GFP when there is uric acid. In BBa_K2197301, the expression of GFP is carried out by this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 723